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antibody against por  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology antibody against por
    Antibody Against Por, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 93/100, based on 55 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antibody+against+por/Porfiromycin/pmc09481905__mmc2-282-11-15
    Average 93 stars, based on 55 article reviews
    antibody against por - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Membrane:

    Article Title: P450 oxidoreductase regulates barrier maturation by mediating retinoic acid metabolism in a model of the human BBB
    Article Snippet: The membrane was stained with Ponceau S (Sigma) to ensure equal protein loading, then washed with tris buffered saline with tween-20 (TBST, 20mM Tris, 150 mM NaCl, 0.1% Tween-20), and blocked for 1 hour in 5% BSA in TBST at RT. .. Next, the membrane was incubated overnight at 4°C with a primary antibody against POR (1:500, Santa-Cruz) in TBST with 1% BSA. .. Anti-mouse secondary antibodies conjugated with horseradish peroxidase (1:5000, Santa-Cruz) was applied in TBST with 2.5% BSA for 1 hour at RT followed by chemiluminescence imaging using Westar Supernova (Cyanagen) in Fusion Solo X (Vilber, Collegien, France) imaging system.

    Article Title: Supplemental Information P450 oxidoreductase regulates barriermaturation bymediating retinoic acid metabolism in a model of the human BBB
    Article Snippet: The membrane was stained with Ponceau S (Sigma) to ensure equal protein loading, then washed with tris buffered saline with tween-20 (TBST, 20mM Tris, 150 mM NaCl, 0.1% Tween-20), and blocked for 1 hour in 5% BSA in TBST at RT. .. Next, the membrane was incubated overnight at 4°C with a primary antibody against POR (1:500, Santa-Cruz) in TBST with 1% BSA. .. Anti-mouse secondary antibodies conjugated with horseradish peroxidase (1:5000, Santa-Cruz) was applied in TBST with 2.5% BSA for 1 hour at RT followed by chemiluminescence imaging using Westar Supernova (Cyanagen) in Fusion Solo X (Vilber, Collegien, France) imaging system.

    Incubation:

    Article Title: P450 oxidoreductase regulates barrier maturation by mediating retinoic acid metabolism in a model of the human BBB
    Article Snippet: The membrane was stained with Ponceau S (Sigma) to ensure equal protein loading, then washed with tris buffered saline with tween-20 (TBST, 20mM Tris, 150 mM NaCl, 0.1% Tween-20), and blocked for 1 hour in 5% BSA in TBST at RT. .. Next, the membrane was incubated overnight at 4°C with a primary antibody against POR (1:500, Santa-Cruz) in TBST with 1% BSA. .. Anti-mouse secondary antibodies conjugated with horseradish peroxidase (1:5000, Santa-Cruz) was applied in TBST with 2.5% BSA for 1 hour at RT followed by chemiluminescence imaging using Westar Supernova (Cyanagen) in Fusion Solo X (Vilber, Collegien, France) imaging system.

    Article Title: Supplemental Information P450 oxidoreductase regulates barriermaturation bymediating retinoic acid metabolism in a model of the human BBB
    Article Snippet: The membrane was stained with Ponceau S (Sigma) to ensure equal protein loading, then washed with tris buffered saline with tween-20 (TBST, 20mM Tris, 150 mM NaCl, 0.1% Tween-20), and blocked for 1 hour in 5% BSA in TBST at RT. .. Next, the membrane was incubated overnight at 4°C with a primary antibody against POR (1:500, Santa-Cruz) in TBST with 1% BSA. .. Anti-mouse secondary antibodies conjugated with horseradish peroxidase (1:5000, Santa-Cruz) was applied in TBST with 2.5% BSA for 1 hour at RT followed by chemiluminescence imaging using Westar Supernova (Cyanagen) in Fusion Solo X (Vilber, Collegien, France) imaging system.



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    Santa Cruz Biotechnology antibodies against por
    TPZ cytotoxicity under aerobic conditions displays major bias towards cells with <t>POR</t> localized in the nuclear compartment: ( A ) Sensitivity of parental and transfected MDA-231 cell lines to TPZ under aerobic conditions as determined by antiproliferative assay. IC 50 values show the mean of 2-4 independent experiments, error bars show the SEM; ( B ) Sensitivity of parental and transfected MDA-231 cell lines to TPZ under aerobic conditions as determined by clonogenic assay. C 10 values are derived from a single experiment (triplicate plates per drug concentration); ( C ) Relative metabolic efficiency in MDA-231 transfectants (extent to which productive metabolism contributes to cytotoxicity). M 50 = 1/(IC 50 × k met ) of anoxic TPZ activity as derived from A. M 10 = 1/(C 10 × k met ) of anoxic TPZ activity as derived from A). The M 50 and C 10 values were normalised to the value for MDA-231 WT cells; ( D ) Flow cytometry analysis of γ-H2AX levels in parental and transfected MDA-231 cells following a 4 h aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions. Control is no antibody labelling; ( E ) Flow cytometry analysis of phospho-53bp1 levels in parental and transfected MDA-231 cells following a 4 h aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions. Control is secondary antibody only; ( F ) Immunofluoresence microscopy showing phospho-53bp1 foci formation following a four hour aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions.
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    Image Search Results


    TPZ cytotoxicity under aerobic conditions displays major bias towards cells with POR localized in the nuclear compartment: ( A ) Sensitivity of parental and transfected MDA-231 cell lines to TPZ under aerobic conditions as determined by antiproliferative assay. IC 50 values show the mean of 2-4 independent experiments, error bars show the SEM; ( B ) Sensitivity of parental and transfected MDA-231 cell lines to TPZ under aerobic conditions as determined by clonogenic assay. C 10 values are derived from a single experiment (triplicate plates per drug concentration); ( C ) Relative metabolic efficiency in MDA-231 transfectants (extent to which productive metabolism contributes to cytotoxicity). M 50 = 1/(IC 50 × k met ) of anoxic TPZ activity as derived from A. M 10 = 1/(C 10 × k met ) of anoxic TPZ activity as derived from A). The M 50 and C 10 values were normalised to the value for MDA-231 WT cells; ( D ) Flow cytometry analysis of γ-H2AX levels in parental and transfected MDA-231 cells following a 4 h aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions. Control is no antibody labelling; ( E ) Flow cytometry analysis of phospho-53bp1 levels in parental and transfected MDA-231 cells following a 4 h aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions. Control is secondary antibody only; ( F ) Immunofluoresence microscopy showing phospho-53bp1 foci formation following a four hour aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions.

    Journal: Molecules

    Article Title: Subcellular Location of Tirapazamine Reduction Dramatically Affects Aerobic but Not Anoxic Cytotoxicity

    doi: 10.3390/molecules25214888

    Figure Lengend Snippet: TPZ cytotoxicity under aerobic conditions displays major bias towards cells with POR localized in the nuclear compartment: ( A ) Sensitivity of parental and transfected MDA-231 cell lines to TPZ under aerobic conditions as determined by antiproliferative assay. IC 50 values show the mean of 2-4 independent experiments, error bars show the SEM; ( B ) Sensitivity of parental and transfected MDA-231 cell lines to TPZ under aerobic conditions as determined by clonogenic assay. C 10 values are derived from a single experiment (triplicate plates per drug concentration); ( C ) Relative metabolic efficiency in MDA-231 transfectants (extent to which productive metabolism contributes to cytotoxicity). M 50 = 1/(IC 50 × k met ) of anoxic TPZ activity as derived from A. M 10 = 1/(C 10 × k met ) of anoxic TPZ activity as derived from A). The M 50 and C 10 values were normalised to the value for MDA-231 WT cells; ( D ) Flow cytometry analysis of γ-H2AX levels in parental and transfected MDA-231 cells following a 4 h aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions. Control is no antibody labelling; ( E ) Flow cytometry analysis of phospho-53bp1 levels in parental and transfected MDA-231 cells following a 4 h aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions. Control is secondary antibody only; ( F ) Immunofluoresence microscopy showing phospho-53bp1 foci formation following a four hour aerobic exposure to 20 µM TPZ and a 1 h drug-free recovery period under aerobic conditions.

    Article Snippet: 30 μg of protein was loaded on SDS-polyacrylamide electrophoresis gels (4–12% gradient gels; Invitrogen, Carlsbad, CA, USA), transferred, blocked, and probed with primary antibodies against POR (Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA).

    Techniques: Transfection, Clonogenic Assay, Derivative Assay, Concentration Assay, Activity Assay, Flow Cytometry, Control, Microscopy